Coding

Part:BBa_I716356:Design

Designed by: Austin Day   Group: iGEM07_Berkeley_UC   (2007-10-24)


Biobricked Mutant Hemoglobin Cassette in petDUET format


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes


This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.


Source

Pre-biobricking modifications to I716093. Quickchange AD0013F/R to get rid of EcoR1 site. Quickchange AD0014/F/R to get rid of BglII site. Quickchange AD0015F/R to get rid of XhoI site.

PCR AD0016F/R on modified I716093. Digest Bgl2/Xho1 and insert into 9145 plasmid. Product is I716356

References